Preparation of sera for subtyping of influenza a viruses by immunofluorescence
Identifieur interne : 000197 ( 1957/Analysis ); précédent : 000196; suivant : 000198Preparation of sera for subtyping of influenza a viruses by immunofluorescence
Auteurs : Mats E. Johansson [Suède] ; Monica Grandien [Suède] ; Leonida Arro [Suède]Source :
- Journal of Immunological Methods [ 0022-1759 ] ; 1979.
English descriptors
- Teeft :
- Affinity chromatography, Allantoic, Allantoic fluid virus suspension, Antibody titers, Antigenic determinants, Antiserum, Appropriate strains, Centrifugation, Clinical specimens, Complete adjuvant, Hemagglutinin, Heterologous, Heterologous immunofluorescence reactivity, High titers, Hswln1, Immunization, Immunoadsorbent columns, Immunofluorescence, Influenza, Influenza antigens, Influenza type, Influenza virions, Johansson, Lectin column, Matrix protein, Nasopharyngeal secretions, Peplomers, Present study, Rapid virus diagnosis, Sheep conjugate, Subtyping, Sucrose gradients, Type antibodies, Type antiserum, Virion, Virion serum, Virus, Virus antibodies, Virus antisera.
Abstract
Abstract: The conditions for preparation of type-specific and subtype-specific influenza A virus reagents to be used in the immunofluorescence technique have been evaluated.Type A-specific antibodies were prepared by passing an antivirion hyperimmune serum through an immunoadsorbent column containing antigens from disrupted virions of a different influenza A virus subtype. The type-specific antibodies were recovered from the immunoadsorbent by desorption with 3 M NaI.For subtype determination, antisera against the various hemagglutinins were used. Such sera could be prepared by removal of irrelevant influenza A virus antibodies from sera directed against purified virions and isolated peplomers, respectively. This was performed by passing the antisera through immunoadsorbent columns containing antigens from disrupted virions of appropriate strains. However, attempts to obtain a hemagglutinin-specific antiserum from a serum directed against allantoic fluid virus suspension failed with this procedure. Antisera obtained after immunization with purified hemagglutinin were also elaborated. These sera were shown to be superior for subtyping of influenza A virus infections by immunofluorescence, but could not a priori be regarded as subtype-specific.The usefulness of subtype-specific sera has been demonstrated on clinical specimens for rapid virus diagnosis.
Url:
DOI: 10.1016/0022-1759(79)90223-0
Affiliations:
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Johansson</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Virology and Department of Vaccine Production, National Bacteriological Laboratory, S-105 21 Stockholm</wicri:regionArea><wicri:noRegion>S-105 21 Stockholm</wicri:noRegion></affiliation></author><author><name sortKey="Grandien, Monica" sort="Grandien, Monica" uniqKey="Grandien M" first="Monica" last="Grandien">Monica Grandien</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Virology and Department of Vaccine Production, National Bacteriological Laboratory, S-105 21 Stockholm</wicri:regionArea><wicri:noRegion>S-105 21 Stockholm</wicri:noRegion></affiliation></author><author><name sortKey="Arro, Leonida" sort="Arro, Leonida" uniqKey="Arro L" first="Leonida" last="Arro">Leonida Arro</name><affiliation wicri:level="1"><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Virology and Department of Vaccine Production, National Bacteriological Laboratory, S-105 21 Stockholm</wicri:regionArea><wicri:noRegion>S-105 21 Stockholm</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Immunological Methods</title><title level="j" type="abbrev">JIM</title><idno type="ISSN">0022-1759</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1979">1979</date><biblScope unit="volume">27</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="263">263</biblScope><biblScope unit="page" to="272">272</biblScope></imprint><idno type="ISSN">0022-1759</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1759</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Affinity chromatography</term><term>Allantoic</term><term>Allantoic fluid virus suspension</term><term>Antibody titers</term><term>Antigenic determinants</term><term>Antiserum</term><term>Appropriate strains</term><term>Centrifugation</term><term>Clinical specimens</term><term>Complete adjuvant</term><term>Hemagglutinin</term><term>Heterologous</term><term>Heterologous immunofluorescence reactivity</term><term>High titers</term><term>Hswln1</term><term>Immunization</term><term>Immunoadsorbent columns</term><term>Immunofluorescence</term><term>Influenza</term><term>Influenza antigens</term><term>Influenza type</term><term>Influenza virions</term><term>Johansson</term><term>Lectin column</term><term>Matrix protein</term><term>Nasopharyngeal secretions</term><term>Peplomers</term><term>Present study</term><term>Rapid virus diagnosis</term><term>Sheep conjugate</term><term>Subtyping</term><term>Sucrose gradients</term><term>Type antibodies</term><term>Type antiserum</term><term>Virion</term><term>Virion serum</term><term>Virus</term><term>Virus antibodies</term><term>Virus antisera</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The conditions for preparation of type-specific and subtype-specific influenza A virus reagents to be used in the immunofluorescence technique have been evaluated.Type A-specific antibodies were prepared by passing an antivirion hyperimmune serum through an immunoadsorbent column containing antigens from disrupted virions of a different influenza A virus subtype. The type-specific antibodies were recovered from the immunoadsorbent by desorption with 3 M NaI.For subtype determination, antisera against the various hemagglutinins were used. Such sera could be prepared by removal of irrelevant influenza A virus antibodies from sera directed against purified virions and isolated peplomers, respectively. This was performed by passing the antisera through immunoadsorbent columns containing antigens from disrupted virions of appropriate strains. However, attempts to obtain a hemagglutinin-specific antiserum from a serum directed against allantoic fluid virus suspension failed with this procedure. Antisera obtained after immunization with purified hemagglutinin were also elaborated. These sera were shown to be superior for subtyping of influenza A virus infections by immunofluorescence, but could not a priori be regarded as subtype-specific.The usefulness of subtype-specific sera has been demonstrated on clinical specimens for rapid virus diagnosis.</div></front></TEI><affiliations><list><country><li>Suède</li></country></list><tree><country name="Suède"><noRegion><name sortKey="Johansson, Mats E" sort="Johansson, Mats E" uniqKey="Johansson M" first="Mats E." last="Johansson">Mats E. Johansson</name></noRegion><name sortKey="Arro, Leonida" sort="Arro, Leonida" uniqKey="Arro L" first="Leonida" last="Arro">Leonida Arro</name><name sortKey="Grandien, Monica" sort="Grandien, Monica" uniqKey="Grandien M" first="Monica" last="Grandien">Monica Grandien</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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